Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template....

C5L2, also known as GPR77, is a 45kD 7TM receptor that binds the complement proteins , C3a, C4a, and C5a as well as the acylation stimulating peptide (ASP). C5L2 is expressed on , various hematopoietic cells where it promotes complement-mediated inflammation. ASP , binding to C5L2 on adipocytes triggers triglyceride...